Journal:

Article Title: Loss of desmoplakin isoform I causes early onset cardiomyopathy and heart failure in a Naxos-like syndrome

doi: 10.1136/jmg.2005.032904

Figure Lengend Snippet: Figure 4 DSP isoform distribution in human heart. Specific amplification of DSPI, DSPII, and control GDH on a cardiovascular cDNA panel from various compartments of the human adult and fetal heart. Additional amplification of skin cDNA was used as an internal control.

Article Snippet: AT, annealing temperature; WT, wild type. caption a8 Table 1 Primers used for PCR on cardiovascular cDNA panel PCR was carried out using cDNA from the Clontech human cardiovascular multiple tissue cDNA panel (BD Biosciences) in a total volume of 25 µl containing 0.5 ng cDNA.

Techniques: Amplification

Journal: Molecular and Cellular Biology

Article Title: Mice with Deficiency of G Protein ? 3 Are Lean and Have Seizures

doi: 10.1128/MCB.24.17.7758-7768.2004

Figure Lengend Snippet: (A) RT-PCR products obtained after 30, 34, or 38 cycles of amplification with primers JR282 and JR286, showing relative expression of Gng3 in various tissues (top three panels), or products from 24 cycles with primers for glyceraldehyde-3-phosphate dehydrogenase (Gapd), confirming approximately equal amounts of cDNA in all reactions (bottom panel). (B) (Top) RT-PCR products obtained after 30 cycles of amplification with primers JR282 and JR286 for Gng3, from cDNA prepared from whole brains of three wild-type, three Gng3+/−, and three Gng3−/− mice, without added reverse transcriptase (−RT), or without added cDNA (−cDNA). (Bottom) RT-PCR products obtained after 29 cycles of amplification with primers JR174 and JR175 for elongation factor 1 α2 (Eef1a2), from identical aliquots of the cDNAs. (C) Western blot analysis of proteins prepared from Sf9 cells expressing γ3 (Std) or brains of three Gng3−/−, three Gng3+/−, and three wild-type mice. Top panel shows that γ3 is reduced in membranes from Gng3+/− mice and absent in membranes from Gng3−/− mice. Bottom panel shows that sodium/potassium ATPase β-subunit levels are equal in all lanes.

Article Snippet: PCR amplification of cDNA in the murine multiple tissue cDNA panel (BD Biosciences, Palo Alto, Calif.) was performed according to the manufacturer's instructions.

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Expressing, Western Blot

Journal: Molecular and Cellular Biology

Article Title: Mice with Deficiency of G Protein ? 3 Are Lean and Have Seizures

doi: 10.1128/MCB.24.17.7758-7768.2004

Figure Lengend Snippet: (A) Organization of the Bscl2 and Gng3 genes, showing the first few exons (exons 1 to 3) of Bscl2 (shaded boxes), including the alternate exon 1 (1a), and the three exons of Gng3 (solid boxes). The region replaced in the targeted allele, Gng3−, is indicated by the double arrow. Arrows mark positions of primers used for amplification of Bscl2: the antisense primer JR443 (a), the exon 1-specific sense primer JR445 (b), and the alternate exon 1-specific sense primer JR446 (c). (B) Duplex RT-PCR of brain cDNA from three Gng3−/− (−/−), three Gng3+/− (+/−), and three wild-type (+/+) mice, with primers for exon 1 of Bscl2 (upper panel), the alternate exon 1 of Bscl2 (lower panel), and elongation factor Eef1a2 (both panels), showing approximately equal levels of either Bscl2 transcript across all genotypes.

Article Snippet: PCR amplification of cDNA in the murine multiple tissue cDNA panel (BD Biosciences, Palo Alto, Calif.) was performed according to the manufacturer's instructions.

Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction